Bio-Spin® Chromatography Columns from Bio-Rad

Product Specs
ItemBio-Spin® Chromatography Columns
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number7326008
QuantityPkg of 100
Brand/TrademarkBio-Rad Laboratories, Life Science Group
TypeColumns

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) A Single-Step Immunocapture Assay to Quantify HCC Exosomes Using the Highly Sensitive Fluorescence Nanoparticle-Tracking AnalysisJournal of Hepatocellular CarcinomaNovember 2, 2023Ali Riza Koksal, Nergiz Ekmen, Yucel Aydin, Kelley Nunez, Tyler Sandow, Molly Delk, Martin Moehlen, Paul Thevenot, Ari Cohen, Srikanta DashSephadex G-200 (“hand-made gel column”, A 50120, Sigma, Burlington MA), Bio-Spin Chromatography Columns (7326008 Bio-Rad, Hercules, CA), Cell guidance Exo-spin mini columns (EX03-50), and Amersham MicroSpin G-50 Columns

(2) Neuronal Ndst1 depletion accelerates prion protein clearance and slows neurodegeneration in prion infectionPLOS PathogensSeptember 25, 2023Patricia Aguilar-Calvo, Adela Malik, Daniel R. Sandoval, Christopher Barback, Christina D. Orrù, Heidi G. Standke, Olivia R. Thomas, Chrissa A. Dwyer, Donald P. Pizzo, Jaidev Bapat, et al.affinity chromatography, Heparin Sepharose 6 Fast Flow beads (Healthcare Life Sciences) were loaded into Bio-Spin chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES, pH 7.4). Supernatants

(3) The Impact of ETV6-NTRK3 Oncogenic Gene Fusions on Molecular and Signaling Pathway AlterationsCancers (Basel)August 24, 2023Matias Kinnunen, Xiaonan Liu, Elina Niemelä, Tiina Öhman, Lisa Gawriyski, Kari Salokas, Salla Keskitalo, Markku Varjosalostrep-tactin sepharose beads (IBA Lifesciences #2-1201-025, Göttingen, Germany) in spin columns (Bio-Rad #7326008, Hercules, CA, USA). A 200 μL volume of strep-tactin beads were prewashed with 1ml of lysis buffer.

(4) Calcineurin associates with centrosomes and regulates cilia length maintenanceJournal of Cell ScienceApril 15, 2023Eirini Tsekitsidou, Cassandra J. Wong, Idil Ulengin-Talkish, Angela I. M. Barth, Tim Stearns, Anne-Claude Gingras, Jennifer T. Wang, Martha S. Cyerth, and the beads were then isolated and eluted through a Bio-Spin Chromatography Column (Bio-Rad, #7326008) with elution buffer (50 mM Tris-HCl pH 8, 300 mM NaCl, 0.1% NP-40, 5 mM DTT and 40 mM glutathione;

(5) Comprehensive characterization of the embryonic factor LEUTXiScienceMarch 17, 2023Lisa Gawriyski, Eeva-Mari Jouhilahti, Masahito Yoshihara, Liangru Fei, Jere Weltner, Tomi T. Airenne, Ras Trokovic, Shruti Bhagat, Mari H. Tervaniemi, Yasuhiro Murakawa, et al.201388,https://github.com/alexdobin/STAR Cutadapt v 1.1.8,Martin 2011,http://code.google.com/p/cutadapt/ ,, Other,Other,Other ,, Bio-Spin Chromatography Columns,Bio-Rad,Cat# 732-6008 100-mm-long reversed-phase C18 end-capped HPLC column,Merck,Cat# 1021290001 Autosampler

(6) The chromatin factor ROW cooperates with BEAF ‐32 in regulating long‐range inducible genesEMBO reportsDecember 6, 2022Neta Herman, Sebastian Kadener, Sagiv ShifmanmM Spermine and protease inhibitor tablets [Roche]) for a total of 2 min. Homogenate was poured into Bio‐Spin chromatography columns (BIO‐RAD) and centrifuged at 1,000 g for 4 min. The filtered homogenate was centrifuged at 6,000 g for

(7) The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistanceNature CommunicationsDecember 1, 2022Ian D. Ferguson et al.were washed three times with 1 mL 2X RIPA/1 mM EDTA buffer in 2-mL chromatography columns (Bio-Rad 7326008) attached to a vacuum manifold (Promega A7231). Clarified lysates were then transferred to the chromatography

(8) Physical and functional interactome atlas of human receptor tyrosine kinasesEMBO reportsJune 7, 2022Salokas K, Liu X, Öhman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M.bicarbonate (AMBIC),Sigma‐Aldrich,1066‐33‐7 Benzonase® Nuclease,Santa Cruz Biotechnology,sc‐202391 Bio‐Spin® Chromatography Columns,Bio‐Rad,732‐6008 Biotin,Thermo Fisher Scientific,29129 Bradford reagent,Bio‐Rad,500‐0006 DAPI,Sigma‐Aldrich,Cat#

(9) Tubulin/microtubules as novel clozapine targetsNeuropsychopharmacology ReportsMarch 1, 2022Mizuki Hino, Takeshi Kondo, Yasuto Kunii, Junya Matsumoto, Akira Wada, Shin‐ichi Niwa, Mitsutoshi Setou, Hirooki YabeNeutrAvidin agarose beads (29201, Thermo, Tokyo, Japan) for 16 h and transferred into the spin column (7326008, Bio‐Rad, Tokyo, Japan). Then, the 1 ml of soluble fractions was added to each column and incubated

(10) Bidirectional protein–protein interactions control liquid–liquid phase separation of PSD-95 and its interaction partnersiScienceFebruary 18, 2022Nikolaj Riis Christensen, Christian Parsbæk Pedersen, Vita Sereikaite, Jannik Nedergaard Pedersen, Maria Vistrup-Parry, Andreas Toft Sørensen, Daniel Otzen, Kaare Teilum, Kenneth Lindegaard Madsen, Kristian Strømgaardthis step was repeated twice, followed by transfer of beads to a single use gravity column (BioRad 7326008), followed by three on column washes. The protein was eluted using 10 mM reduced GSH (Sigma G4251) in

(11) SARS-CoV-2-host proteome interactions for antiviral drug discoveryMolecular systems biologyNovember 1, 2021Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, Pöhner I, Öhman T, Tonduru AK, Hassinen A, et al.Trypsin,Promega,V5113 Strep‐Tactin® Sepharose® 50% (vol/vol) suspension,IBA life sciences,2‐1201‐010 Bio‐Spin® Chromatography Columns,Bio‐Rad,732‐6008 Sodium fluoride,Sigma,7681‐49‐4 Protease inhibitor cocktail,Sigma‐Aldrich,P8340/P2745 Biotin,Thermo Fisher Scientific

(12) The SZT2 Interactome Unravels New Functions of the KICSTOR ComplexCellsOctober 9, 2021Cattelani C, Lesiak D, Liebscher G, Singer II, Stasyk T, Wallnöfer MH, Heberle AM, Corti C, Hess MW, Pfaller K, et al.µg/mL Pepstatin) and transferred into one pre-equilibrated ‘Bio-Spin Disposable Chromatography Column’ (7326008 Biorad, Hercules, CA, USA) per sample. Then, the columns were washed 6 times with washing buffer followed

(13) Top-down mass spectrometry of histone modifications in sorghum reveals potential epigenetic markers for drought acclimationMethodsDecember 1, 2020Mowei Zhou et al.Sigma-Aldrich. BioRex70 weak cation exchange resin (mesh 100–200 analytical, catalog # 1425842) and Bio-Spin chromatography columns (catalog # 732-6008) were purchased from BioRad. Disposable items used included: conical centrifuge

(14) Combined proximity labeling and affinity purification−mass spectrometry workflow for mapping and visualizing protein interaction networksNature ProtocolsOctober 1, 2020Xiaonan Liu, Kari Salokas, Rigbe G. Weldatsadik, Lisa Gawriyski, Markku VarjosaloA69060250) ● Strep-Tactin Sepharose 50% (vol/vol) suspension (IBA Life Sciences, cat. no. 2-1201-010) ● Bio-Spin Chromatography Columns (Bio-Rad, cat. no. 732-6008) ● Ethylenediaminetetraacetic acid disodium salt (EDTA; Chemsupply, cat.

(15) Karyopherin-β2 Inhibits and Reverses Aggregation and Liquid-liquid Phase Separation of the ALS/FTD-Associated Protein FUSBIO-PROTOCOLAugust 20, 2020Emma Robinson, James Shorter, Lin Guo(Carl Zeiss, catalog number: 12-070-397) 34. Bio-spin Chromatography Columns (Bio-Rad, catalog number: 7326008) 35. HiTrap-Q anion exchange chromatography column (GE Healthcare, catalog number: 17115301) 36. IPTG

(16) Nanosphere Loaded with Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificusCellsMarch 5, 2020Kim JY, Lee YM, Kim DW, Min T, Lee SJ.with ampicillin. The cells mixed with Ni-NTA agarose beads (Qiagen, Valencia, CA, USA) were loaded on Bio-Spin Chromatography Columns (Bio-Rad Laboratories, Hercules, CA, USA). The eluted VvhA protein was dialyzed with Slide-A-Lyzer Dialysis

(17) Physical and functional interaction between SET1/COMPASS complex component CFP-1 and a Sin3S HDAC complex in C. elegansNucleic Acids ResearchDecember 2, 2019Flore Beurton et al.approximately 60 mg of total protein extract was incubated with protein G agarose beads [Sigma-Aldrich] in Bio-Spin Chromatography columns [Bio-Rad] for 30 min at 4°C on a rotator. Flow-through was collected and incubated with 240 μl slurry

(18) Dominant TOM1 mutation associated with combined immunodeficiency and autoimmune diseasenpj Genomic MedicineDecember 1, 2019Salla Keskitalo et al.(Santa Cruz Biotechnology) in HNN lysis buffer. The proteins were bound using Strep-Tactin sepharose and Bio-Spin chromatography columns (Bio-Rad) and eluted with D-biotin (Thermo Scientific). After C18-purification samples were analyzed

(19) Co-opting the fermentation pathway for tombusvirus replication: Compartmentalization of cellular metabolic pathways for rapid ATP generationPLOS PathogensOctober 24, 2019Wenwu Lin, Yuyan Liu, Melissa Molho, Shengjie Zhang, Longshen Wang, Lianhui Xie, Peter D. Nagyinhibitor cocktail). The supernatant was incubated with anti-FLAG M2 affinity agarose (Sigma-Aldrich) using Bio-spin chromatography columns (Bio-rad) for 2 h at 4°C on a rotator, followed by washing with the CP buffer (10% [v/v] glycerol, 25

(20) Cyclin B3 promotes anaphase I onset in oocyte meiosisJournal of Cell BiologyApril 1, 2019Mehmet E. Karasu, Nora Bouftas, Scott Keeney, Katja WassmannResin was washed briefly with ice-cold lysis buffer before transfer on Bio-Spin chromatography columns (7326008; Bio-Rad). The resin on the column was washed five times with ice-cold wash buffer (lysis buffer plus

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